Fig. 4
Diagram of culture setup. Tissue culture media (red) is placed in a reservoir bubbled with 99% O2 gas. A feeding peristaltic pump is used to pull media out of the reservoir into the culture device bottom (B), bathing the explant (orange form). A vertical wall in the bottom portion of the chamber maintains the depth of the liquid media at the tissue support grid A recovery pump is used to pull the media out of the device back into the media reservoir. A gas mixture of choice (green) flows with low pressure into the top part of the device (A) from a compressed source